Phenotype Image Detail

Image

Caption

A: A normal Kif3a^tm1Gsn/Kif3a⁺ (+/-) embryo at 9.5pc displaying proper cardiac looping. B: A Kif3a^tm1Gsn/Kif3a⁺ embryo exhibiting retarded cardiac looping. C: A Kif3a^tm1Gsn/Kif3a^tm1Gsn (-/-) embryo displaying reversed cardiac looping. Arrowheads in A-C indicate direction of cardiac looping. D-F: Closeup of hearts in A-C. G & H: Right and left side views of homozygous mutant embryo with reversed cardiac looping. I & J: A wild type embryo (I) that underwent normal embryo turning and a homozygous mutant embryo (J) that failed to undergo embryonic turning. Arrow indicates the orientation of the embryo posterior. K: Homozygous mutant embryo exhibiting edema around the heart. Blue arrowheads in B & K indicate the membrane. L: Homozygous mutant embryo that has a neural tube closure defect. Large arrowheads point to open neural tube and small arrowheads point to closed neural tube. M-O: In situ hybridization of 8.0pc expression of Pitx2 in the lateral plate mesoderm in wild-type embryos. N & O: Bilateral expression of Pitx2 in homozygous mutant embryos. Arrowheads point to strong lateral plate mesoderm staining.

Copyright

This image is from Marszalek JR, Proc Natl Acad Sci U S A 1999 Apr 27;96(9):5043-8. Copyright 1999 National Academy of Sciences, U.S.A. J:54575

Associated Alleles

Symbol	Name
Kif3a^tm1Gsn	kinesin family member 3A; targeted mutation 1, Lawrence SB Goldstein

Associated Genotypes

Allelic Composition	Genetic Background
Kif3a^tm1Gsn/Kif3a^tm1Gsn	involves: 129S1/Sv * 129X1/SvJ
Kif3a^tm1Gsn/Kif3a^+	involves: 129S1/Sv * 129X1/SvJ