Phenotype Image Detail

Image

Caption

A-D: Whole-mount immunofluorescence staining of a wild type (A) and Flnb^{Gt(BGB085)Byg}/ Flnb^{Gt(BGB085)Byg} (B) embryo with anti-CD31 antibody at E11.5 (red). The vascular network around the vertebral column and in the central nervous system is indicated (white arrows) to demonstrate an impaired vascularization in the homozygous mutant embryo at E11.5. C & D: An area of these embryos is enlarged to compare the vascular patterning. Note the proper sprouting of microvasculature in the wild type mouse embryo (C, small white arrows), which is severely impaired in the homozygous mutant embryo. E & F: Histological pictures of the central nervous system stained with hematoxylin/eosin of wild type (E) and homozygous mutant (F) mouse embryos at E11.5. G & H: Middle cerebral artery between midbrain (mb), diencephalon (de), and trigeminal ganglion (tg) is enlarged. In this area of the homozygous mutant embryo, note that the middle cerebral artery is rudimentary (al also indicated in A, right arrow), and endothelial cells covering small vascular structures express LacZ (H Inset). (I and J) Embryological sections stained with an anti-CD31 antibody demonstrate impaired and disorganized vascular network around the vertebral column at E13.5.

Copyright

This image is from Zhou X, Proc Natl Acad Sci U S A 2007 Mar 6;104(10):3919-24. Copyright 2007 National Academy of Sciences, U.S.A. J:120066

Associated Alleles

Symbol	Name
Flnb^Gt(BGB085)Byg	filamin, beta; gene trap BGB085, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Flnb^Gt(BGB085)Byg/Flnb^Gt(BGB085)Byg	involves: 129P2/OlaHsd * C57BL/6