Phenotype Image Detail

Image

Caption

Corneal stroma in wild type (left) and Chst5^tm1Knsh/Chst5^tm1Knsh mice (right), showing PGs stained with Cupromeronic blue after incubation in buffer (A and D), keratanase (B and E), and chondroitinase ABC (C and F). Small collagen-associated PG filaments remaining after chondroitinase ABC digestion in the wild type cornea (C, arrowheads) represent sulfated KSPGs. These are not present in homozygous mutant cornea (F). Abnormally large, caterpillar-like PGs (arrows), not present in the wild type stroma (A), are evident in the mutant cornea (D). These are susceptible to chondroitinase ABC (F), but not to keratanase (E), digestion pointing to a significant CS/DS component. Scale bar 300nm

Copyright

This image is from Hayashida Y, Proc Natl Acad Sci U S A 2006 Sep 5;103(36):13333-8. Copyright 2006 National Academy of Sciences, U.S.A. J:112899

Associated Alleles

Symbol	Name
Chst5^tm1Knsh	carbohydrate sulfotransferase 5; targeted mutation 1, Kohji Nishida

Associated Genotypes

Allelic Composition	Genetic Background
Chst5^tm1Knsh/Chst5^tm1Knsh	B6.129-Chst5^tm1Knsh