Phenotype Image Detail

Image

Caption

A-F: Transmission electron microscopic features of spermatogenic cells. The panels to the left ( A, C & E) show sections from wild type mice; those to the right (B,D & F) are from Gopc^tm1.1Tno/Gopc^tm1.1Tno mice. A: In step 6 spermatids in wild type mice, a cap-like acrosome was formed around the nucleus as indicated by an arrow. Ac, acrosome; Nu, nucleus. B: In step 6 spermatids in homozygous mutant mice, the developing acrosome is fragmented, as indicated by arrows, and ill-developed or abnormally large vesicles which are not observed in wild type spermatid were apparent (Insert). C: In elongating spermatids in wild type mice, chromosomes become condensed, nuclei become elongated, and acrosomes expand around the nucleus. D: In homozygous mutant spermatids, although chromosome condensation is observed, nuclei are still ovoid. The arrowhead points to the vacuolized nucleus. Abnormalities of acrosomes including vacuolization, loss of adhesion to the nucleus, and total loss are apparent. E: Wild type spermatozoa exhibit the characteristic hooked shape. F: In homozygous mutant epididymal spermatozoa, acrosomes are lost and nuclei are ovoid. The arrowhead points to the vacuolized nucleus. An abnormal arrangement of mitochondria in the mitochondrial sheath is also evident (Insert). G & H: Immunoelectron microscopic analysis of a round spermatid. GOPC is localized at the Golgi apparatus (G). Magnification of the rectangular region in G indicates the GOPC protein to be localized in trans-Golgi cisternae and the trans-Golgi network. Note that no signals were detected in the acrosome (Ac) (G), and GOPC proteins were often detected in the medulla of round spermatids, which were apparently associated with vesicle structures as indicated by arrows (H).

Copyright

This image is from Yao R, Proc Natl Acad Sci U S A 2002 Aug 20;99(17):11211-6. Copyright 2002 National Academy of Sciences, U.S.A. J:78599

Associated Alleles

Symbol	Name
Gopc^tm1.1Tno	golgi associated PDZ and coiled-coil motif containing; targeted mutation 1.1, Tetsuo Noda

Associated Genotypes

Allelic Composition	Genetic Background
Gopc^tm1.1Tno/Gopc^tm1.1Tno	involves: 129S4/SvJae * C57BL/6