Phenotype Image Detail

Image

Caption

Histological appearance of limbs from wild type and Runx2^tm1Gss/Runx2^tm1Gss (deltaC/deltaC) embryos at 17.5dpc. Tibial bone paraffin sections stained with Safranin O-fast green (A-D) from wild type (A & C) and homozygous (B & D) mice at x4 (A & B) and x10 (C & D) magnifications to show growth plate zones. Cartilage (red) and bone, marrow and blood vessels (blue) are distinguished. Brackets in A & B show regions at higher magnification in C & D. Undecalcified tibial bone sections with hematoxylin and eosin/von Kossa silver staining (to detect mineral) for wild type (E) and mutant mice (F) at x10 magnification. Alkaline phosphatase (G) and tartrate-resistant acid phosphatase (H) histochemistry of the mutant mouse bone (x20). The central portion of the limb (I) from the mutant mouse bone indicates accumulation of blood vessels (hematoxylin and eosin stain) (x40). Arrow indicates one osteoclast (Ocl). V designates blood vessels. P show periochondrium.

Copyright

This image is from Choi JY, Proc Natl Acad Sci U S A 2001 Jul 17;98(15):8650-5. Copyright 2001 National Academy of Sciences, U.S.A. J:70461

Associated Alleles

Symbol	Name
Runx2^tm1Gss	runt related transcription factor 2; targeted mutation 1, Gary S Stein

Associated Genotypes

Allelic Composition	Genetic Background
Runx2^tm1Gss/Runx2^tm1Gss	involves: 129S7/SvEvBrd * C57BL/6