Phenotype Image Detail

Image

Caption

A-C: Luxol fast blue (LFB) stained coronal sections of P50.5 littermate control (A), P17.5 (B) and P50.5 (C) Ctsb^tm1Jde/Ctsb^tm1Jde Ctsl^tm1Cptr/Ctsl^tm1Cptr mouse cerebrum demonstrating cortical shrinkage accompanied by white-matter (wm) reduction between days 17 and 50 in double homozygous mutants. (cx, cortex; scale bar in a 1mm). The deep cerebral white matter is not yet myelinated in 17 day mutants (B). D-F: LFB stain of P50.5 wild type (D), P17.5 (E) and P50.5 (F) double homozygous mouse cerebellum (m, molecular layer; g, granule layer; scale bar 1mm). H & I: H & E stain of the above samples at x100. Note the dying bright red Purkinje cell in I, the complete absence of the Purkinje cell layer (p), and the striking reduction in cellular density in the granule cell layer (g) in J. G: Nuclear pyknosis, karyorrhexis, and brightly red-stained angular cytoplasm in dying cortical neurons at P24.5. Healthy neurons exhibit a lightly stained nucleus and a darkly stained nucleolus as seen in the bottom part of G. K: Lipid laden macrophages in the corpus callosum at P50.5 (x1250 in G-K).

Copyright

This image is from Felbor U, Proc Natl Acad Sci U S A 2002 Jun 11;99(12):7883-8. Copyright 2002 National Academy of Sciences, U.S.A. J:77103

Associated Alleles

Symbol	Name
Ctsb^tm1Jde	cathepsin B; targeted mutation 1, Jan Deussing
Ctsl^tm1Cptr	cathepsin L; targeted mutation 1, Christoph Peters

Associated Genotypes

Allelic Composition	Genetic Background
Ctsb^tm1Jde/Ctsb^tm1Jde Ctsl^tm1Cptr/Ctsl^tm1Cptr	involves: 129P2/OlaHsd * C57BL/6J