Phenotype Image Detail

Image

Caption

(A-D) Sagittal sections of forelimb from E15.5 embryos. (A & B) Stained with toluidine blue. (A) Distal ulna from wild type embryo, (B) distal ulna/radius from Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 embryo. Mineralized cartilage is shown by dark purple and osteoid is shown by light blue (red asterisk). (C & D) Humeri sections were hybridized with digoxigenin labeled riboprobe for CalI. (C) Section of wild type humerus from E15.5 embryo, (D) from Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 embryo. (E-H) Sagittal sections of zeugopod from E17.5 embryos (E and F) stained with toluidine blue while G and H are stained for TRAP (brown stain, red arrow). (E and G) E17.5 proximal radius from wild-type embryo, (F and H) E17.5 proximal ulna/radius from Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 embryo. Although vascularization occurs in the absence of BMP2 and BMP4, few osteoclasts are observed in the mineralized cartilage in Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 mice. (I & J) Sections of newborn proximal radius (I) from control animal and newborn proximal ulna/radius (J) from Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 mouse stained with toluidine blue. Red arrow shows the bone marrow cavity in (E) and (I). Note that bone collar (red *) forms at the right time and place in the absence of BMP2 and BMP4. Also note that since Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0 mice occaisionally have only one bone in zeugopod and the elbow joint is occasionally fused, it is difficult to distinguish between the ulna and the radius.

Copyright

This image is from Bandyopadhyay A, PLoS Genet 2006 Dec 22;2(12):e216, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:118257

Associated Alleles

Symbol	Name
Bmp2^tm1Cjt	bone morphogenetic protein 2; targeted mutation 1, Clifford J Tabin
Bmp4^tm1Jfm	bone morphogenetic protein 4; targeted mutation 1, James F Martin
Tg(Prrx1-cre)1Cjt	transgene insertion 1, Clifford J Tabin

Associated Genotypes

Allelic Composition	Genetic Background
Bmp2^tm1Cjt/Bmp2^tm1Cjt Bmp4^tm1Jfm/Bmp4^tm1Jfm Tg(Prrx1-cre)1Cjt/0	involves: 129S4/SvJaeSor * C57BL/6 * SJL