Phenotype Image Detail

Image

Caption

Retina cross sections (20um thick) from wild type (a) and Slc4a3^tm1Ges/Slc4a3^tm1Ges (b) mice stained with hematoxylin and eosin scale bar = 50um. Electron micrographs of wild type (c) and homozygous mutant (d) mouse retinas, scale bar = 5um; OS, outer segment; IS, inner segment; ONL, Outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cells layer. Fundus photos in 8 month old wild type (e) and mutant littermate mouse (f). Confocal microscopic pictures of retina flat mounts stained with GFAP (g, h), scale bar =50um. Confocal microscopic reconstructions (z-stack of 50um depth) of fluorescein filled blood vessels in retina flat mounts showing several loops indicative of shunting between venule and arteriole (arrows) in 8 month old mutant (k) not seen in wild type (i); at higher magnification, tortuous vessels are obvious in mutants (j) compared with straight trajectories in wild type (l), scale bar = 1--um. Confocal fluorescence image of six month old mutant mouse retina (m), stained with anti-glial fibrilar acidic protein (GFAP) antibody, at the level of the optic nerve head (anterior lamina cribosa), scale bar = 50um.

Copyright

This image is from Alvarez BV, PLoS ONE 2007;2(9):e839, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:129363

Associated Alleles

Symbol	Name
Slc4a3^tm1Ges	solute carrier family 4 (anion exchanger), member 3; targeted mutation 1, Gary E Shull

Associated Genotypes

Allelic Composition	Genetic Background
Slc4a3^tm1Ges/Slc4a3^tm1Ges	involves: 129X1/SvJ