Phenotype Image Detail

Image

Caption

A-C: At E18.5 double mutant fetuses have small kidneys in sagittal sections, and some have attenuated cortices and medullae. D-G: Sagittal sections of the distal forelimb from E15.5 embryos revealed altered cartilage zonation in the ulna (D, E) and radius (F, G) bones. Yellow lines indicate the transition from distal/low proliferating (above line) to high proliferating (below line) chondrocyte populations as assessed by cellular morphology. H-I: Sagittal section of the skull base of E18.5 embryos demonstrating abnormal fusion at the cartilaginous interface between the basisphenoid (bs) and basioccipital (bo) bones. J-M: Coronal section (at the midbrain level) of the skull base at E18.5 showing abnormal midline fusion of the basisphenoid (bs) bone in the double mutant embryos. Bones depicted in panels K, L and M were from three separate double mutant embryos. Occasionally the pitutiary gland descended into the gap at the midline of double mutant embryos (as indicated by the arrow in panel L). N-P: Representative sections through the proximal tibia of adult females. Arrows indicate regions of retained cartilage in regions that normally consist only of ossified bone. Representative control mice had the Sulf1^{Gt(OST352220)Lex}/Sulf1⁺ Sulf2^{Gt(OST311938)Lex}/Sulf2⁺ genotype in panels A, D, F, H and N and the Sulf1^{Gt(OST352220)Lex}/Sulf1⁺ Sulf2^{Gt(OST311938)Lex}/Sulf2^{Gt(OST311938)Lex} genotype in panel J. Representative double mutant mice are shown in panels B, C, E, G, I, K, L, M, O and P. Abbreviations: bo, basioccipital; bs, basisphenoid; p, pituitary gland. Scale bar: 500um

Copyright

This image is from Holst CR, PLoS ONE 2007;2(6):e575, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:129305

Associated Alleles

Symbol	Name
Sulf1^Gt(OST352220)Lex	sulfatase 1; gene trap OST352220, Lexicon Genetics
Sulf1^Gt(OST352220)Lex	sulfatase 1; gene trap OST352220, Lexicon Genetics
Sulf2^Gt(OST311938)Lex	sulfatase 2; gene trap OST311938, Lexicon Genetics
Sulf2^Gt(OST311938)Lex	sulfatase 2; gene trap OST311938, Lexicon Genetics

Associated Genotypes

Allelic Composition	Genetic Background
Sulf1^Gt(OST352220)Lex/Sulf1^Gt(OST352220)Lex Sulf2^Gt(OST311938)Lex/Sulf2^Gt(OST311938)Lex	involves: 129S5/SvEvBrd * C57BL/6N