Phenotype Image Detail

Image

Caption

Analysis of monoamines and Choline acetyltransferase (Chat) levels in newborn mice. Coronal sections ( A & C) show the tyrosine hydroxylase (TH) and L-aromatic amino acid decarboxylase (AADC) immunostaining, respectively, in the substantia nigra (A9) and ventral tegmental (A10) area of wild type brain. (B & D) Loss of both markers at the same level in coronal sections of Nr4a2^tm1Omc/Nr4a2^tm1Omc mice. (E) Monoamine levels were measured in the striatum of newborn wild type, heterozygous and homozygous mice by HPLC. A complete loss of dopamine levels in the homozygous mice and a significant decrease in the heterozygous mice were detected. The TH activity in the striatum (F) was significantly decreased in the homozygous and heterozygous mice, while choline acetyltransferase activity (G), norepinephrine, and serotonin (E) were unaffected. Scale bars: 100um

Copyright

This image is from Saucedo-Cardenas O, Proc Natl Acad Sci U S A 1998 Mar 31;95(7):4013-8. Copyright 1998 National Academy of Sciences, U.S.A. J:46856

Associated Alleles

Symbol	Name
Nr4a2^tm1Omc	nuclear receptor subfamily 4, group A, member 2; targeted mutation 1, Orla M Conneely

Associated Genotypes

Allelic Composition	Genetic Background
Nr4a2^tm1Omc/Nr4a2^tm1Omc	involves: 129S7/SvEvBrd * C57BL/6
Nr4a2^tm1Omc/Nr4a2^tm1Omc	involves: 129S7/SvEvBrd