Phenotype Image Detail

Image

Caption

Alcian blue staining of deparaffinised sections of jejunum (A, B, C, magnification x200) and ileum (D, E, F, magnification x400) from wild type MF1 (A, D) and mutant F28 CF/1Ztm Cftr^tm1Hgu/Cftr^tm1Hgu (CF/1-Cftr^{TgH(neoim)Hgu})(B,E) and F27 CF/3Ztm Cftr^tm1Hgu/Cftr^tm1Hgu (CF/3-Cftr^{TgH(neoim)Hgu})(C,F) mice showed focal hypertrophy of goblet cells in the ileum of the two mutant mice. Goblet cell counts are given as percentage of all epithelial cells. From each strain two mice and three sections/mouse were counted. Goblet cell count: CF/1=22.7% +/- 2.3 (p=0.112, p-value CF/1 compared to MF1), CF/3=23% +/- 2.8 (p=0.172, p-value CF/3 compared to MF1), MF1=16.8% +/- 1.2. The jejunal sections of the same mice did not show any signs of goblet cell hypertrophy and were similar to wild type MF1 (Goblet cell count: CF/1 = 7.4% +/- 0.4 (p = 0.18, p-value CF/1 compared to MF1), CF/3 = 8.5% +/- 1.2 (p = 0.77, p-value CF/3 compared to MF1), MF1 = 8.8% +/- 0.7.

Copyright

This image is from Charizopoulou N, BMC Genet 2006;7():18, an open-access article, licensee BioMed Central Ltd. J:112752

Associated Alleles

Symbol	Name
Cftr^tm1Hgu	cystic fibrosis transmembrane conductance regulator; targeted mutation 1, MRC Human Genetics Unit

Associated Genotypes

Allelic Composition	Genetic Background
Cftr^tm1Hgu/Cftr^tm1Hgu	CF/1Ztm
Cftr^tm1Hgu/Cftr^tm1Hgu	CF/3Ztm