Phenotype Image Detail

Image

Caption

A: Phenotype of an E11.5 Braf^tm1.1Sva/Braf^tm1.1Sva fetus (lower) and wild type littermate (upper). Mutant embryos are developmentally retarded and smaller than their wild type littermates, and the placentae are less vascularized. B: Placental defects in mutant embryos. Hematoxylin and eosin stained radial sections of E11.5 and E10.5 placentae from wild type and mutant littermates are shown. In the mutant placentae, the spongiotrophoblast (Sp) and labyrinth layer (L) are severely underdeveloped and disorganized compared with wild type. (Top and Bottom) The dotted lines mark the boundaries between the labyrinth and the spongiotrophoblast layer and between the giant cell layer and the maternal decidua (De). (Insets) Higher magnifications. Thick filled arrows, giant cells; open arrows, hemorrhages. (Middle) Labyrinth architecture in wild type and muatnt E11.5 placentae. t, trophoblasts; s, stroma; thin filled arrows, endothelial cells; filled arrowheads, nucleated embryonic RBCs (red blood cells); open arrowheads, maternal RBCs. C: Massive apoptosis in mutant placenta at E11.5 revealed by TUNEL staining. (Lower) Adjacent sections stained with hematoxylin to show tissue structure. (D) proliferating cells (Ki67⁺, brown staining) are present in E10.5 wild type and mutant placentae. Scale bar, 500um.

Copyright

This image is from Galabova-Kovacs G, Proc Natl Acad Sci U S A 2006 Jan 31;103(5):1325-30. Copyright 2006 National Academy of Sciences, U.S.A. J:105998

Associated Alleles

Symbol	Name
Braf^tm1.1Sva	Braf transforming gene; targeted mutation 1.1, Alcino Silva

Associated Genotypes

Allelic Composition	Genetic Background
Braf^tm1.1Sva/Braf^tm1.1Sva	involves: 129S1/Sv * 129T2/SvEms * 129X1/SvJ