Phenotype Image Detail

Image

Caption

Histological analyses of control (Gba^tm2Karl/Gba⁺ Tg(KRT14-cre)8Brn/?) (A-D) and Gba1^tm2Karl/Gba1^tm2Karl Tg(KRT14-cre)8Brn/? mice (E-L). Nissl staining of cryosections of brains of mutant mice displayed normal brain architecture (E) similar to the control mice (A). Extensive loss of neurons was observed in the cortex (F) and thalamus (H). C and G are higher magnifications of the cortical layer V (indicated by squares labeled "C" and "G") from B and F, respectively. Note the loss of large pyramidal neurons in cortical layer V (G). (H) The thalamic region of mutant mice also showed a severe loss of neurons. (I) A large number of pyknotic cells (arrows) were scattered throughout the brain. (J) Neurons surrounded by microglia processes of one or several phagocytic cells with ameboid shapes (arrows) were frequently detected in regions with abundant apoptotic cell death. Note the presence of huge vacuoles (arrows) inside large neurons of the motor trigeminal nucleus (K) and pons region (L) of mutant mice. All mice were about 2 weeks old at analysis. In B and F, I-VI indicate the cortical layers. Scale bars: A, 600um; B, 200um; C, D, K & L, 30um; I, 20um. J, 10um. Scale of E matches A, F matches B, G matches C and H matches D.

Copyright

This image is from Enquist IB, Proc Natl Acad Sci U S A 2007 Oct 30;104(44):17483-8. Copyright 2007 National Academy of Sciences, U.S.A. J:127108

Associated Alleles

Symbol	Name
Gba1^tm2Karl	glucosylceramidase beta 1; targeted mutation 2, Stefan Karlsson
Tg(KRT14-cre)8Brn	transgene insertion 8, Anton Berns

Associated Genotypes

Allelic Composition	Genetic Background
Gba1^tm2Karl/Gba1^tm2Karl Tg(KRT14-cre)8Brn/?	involves: 129S1/Sv * 129X1/SvJ * FVB/N