Phenotype Image Detail

Image

Caption

Immunostaining with microglia markers: Iba-1 (A-L) and mac-2 (M-X) in indicated brain regions of Gba1^tm2Karl/Gba1^tm2Karl Tg(KRT14-cre)8Brn/? (K14-lnl/lnl) mice (A-F and M-R) and Gba1^tm1Karl/Gba1^tm1Karl Tg(Nes-cre)1Kln/? (nestin-flox/flox) (G-L and S-X) mice. Iba-1 stains both resting and activated microglia, whereas mac-2 is more selective for activated/proliferating microglia. No mac-2 staining was observed in control mice. Higher magnification of the thalamic region shows the presence of lipid-engorged microglia (arrows) in the Gba1^tm2Karl/Gba1^tm2Karl Tg(KRT14-cre)8Brn/? mice (E,F,Q and R) but not in Gba1^tm1Karl/Gba1^tm1Karl Tg(Nes-cre)1Kln/? mice (K,L,W and X). Note the higher activation of microglia in Gba1^tm1Karl/Gba1^tm1Karl Tg(Nes-cre)1Kln/? mice. All mice were analyzed at end-stage paralysis, corresponding to an age of 2 to 3 weeks. Scale bars: A, 600um; B-E, 80um; F, 20um. Scale within columns is uniform.

Copyright

This image is from Enquist IB, Proc Natl Acad Sci U S A 2007 Oct 30;104(44):17483-8. Copyright 2007 National Academy of Sciences, U.S.A. J:127108

Associated Alleles

Symbol	Name
Gba1^tm1Karl	glucosylceramidase beta 1; targeted mutation 1, Stefan Karlsson
Gba1^tm2Karl	glucosylceramidase beta 1; targeted mutation 2, Stefan Karlsson
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein

Associated Genotypes

Allelic Composition	Genetic Background
Gba1^tm2Karl/Gba1^tm2Karl Tg(KRT14-cre)8Brn/?	involves: 129S1/Sv * 129X1/SvJ * FVB/N
Gba1^tm1Karl/Gba1^tm1Karl Tg(Nes-cre)1Kln/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL