Phenotype Image Detail

Image

Caption

A-H: Immunohistochemical analysis using GFAP staining on brains from control (A-D) and Gba1^tm2Karl/Gba1^tm2Karl Tg(KRT14-cre)8Brn/? mice (K14-lnl/lnl, E-H). I-P: Microglia staining for the Iba-1 marker in brains of control (I-L) and mutant mice (M-P). Mutant mice display increased GFAP staining in the cortex (F) and hippocampus (G). Pronounced staining was observed within hippocampal regions, CA3, dentate gyrus (DG), and thalamus (H). I and J: Iba-1 staining revealed the homogeneous presence of resting microglia throughout the brain of control mice. In contrast, massive activation of microglia is detected in mice lacking Gba in different brain regions (M). Cortical layer V is the most severely affected cortical region(N). Microglial activation is restricted to CA2, CA3 and DG regions of the hippocampus while the CA1 region is relatively spared (O). The thalamic or the hypothalamic region of mutant mice demonstrated a profound microglia repsonse (P). All mice were about 2 weeks old at analysis. Scale bar: A, E, I and M, 600um; B-D, F-H, J-L and N-P, 200um.

Copyright

This image is from Enquist IB, Proc Natl Acad Sci U S A 2007 Oct 30;104(44):17483-8. Copyright 2007 National Academy of Sciences, U.S.A. J:127108

Associated Alleles

Symbol	Name
Gba1^tm2Karl	glucosylceramidase beta 1; targeted mutation 2, Stefan Karlsson
Tg(KRT14-cre)8Brn	transgene insertion 8, Anton Berns

Associated Genotypes

Allelic Composition	Genetic Background
Gba1^tm2Karl/Gba1^tm2Karl Tg(KRT14-cre)8Brn/?	involves: 129S1/Sv * 129X1/SvJ * FVB/N