Phenotype Image Detail

Image

Caption

Electron micrographs of ribbon synapses in the outer plexiform layer (OPL) from wild type (A and C) and Dnajc5^tm1Sud/Dnajc5^tm1Sud mice (B and D-H) at P28. Low magnification views (A and B) show dense and regular arrangement of ribbon synapses (sy) in the OPL close to the outer nuclear layer (ONL) in wild type mice (A) but not in mutant mice (B) in which degenerated ribbon synaptic complexes are found in the OPL (asterisks). The neuropil in the OPL of mutant mice was increased, with processes (fib) crossing the OPL and sprouting into the ONL (B). High magnification views (C-H) in wild type retina (C) show a typical ribbon synapse with an invaginated presynaptic terminal. In contrast, photoreceptor ribbon synapses from mutant mice displayed a pathological morphology (D-H) with decreased synaptic vesicle density (D-H) with electron-dense aggregates in some terminals (E). The arrowhead in F points to an omega-shaped exocytosis figure in a moderately degenerated ribbon terminal with three docked synaptic vesicles nearby (underlined). In the most severely degenerated presynaptic terminals (F and H), presynaptic terminals are large and electron-lucent with few synaptic vesicles but many coated vesicles (cf. F and H). ONL, outer nuclear layer; sy, ribbon synapse; n, nucleus; m, mitochondria; Inv, invagination of the postsynaptic dendrites into to the presynaptic terminal; cv, coated vesicles; fsr, floating (detached) synaptic ribbon; agg, electron dense aggregate; fib, sprouting process; pr, presynaptic; po, postsynaptic, Scale bars, A-D, 1um; E-H, 500nm.

Copyright

This image is from Schmitz F, Proc Natl Acad Sci U S A 2006 Feb 21;103(8):2926-31. Copyright 2006 National Academy of Sciences, U.S.A. J:107310

Associated Alleles

Symbol	Name
Dnajc5^tm1Sud	DnaJ heat shock protein family (Hsp40) member C5; targeted mutation 1, Thomas C Sudhof

Associated Genotypes

Allelic Composition	Genetic Background
Dnajc5^tm1Sud/Dnajc5^tm1Sud	involves: 129S6/SvEvTac