Phenotype Image Detail

Image

Caption

A: P16 wild type and Tg(KRT14-Snai1)1Efu/0 mice. Insets denote magnified tail segments, which displayed a mosaic, flaky appearance in transgenic mice. Size differences appeared with age, and are likely due to K14-promoter activity in the tongue and oral epithelium, resulting in progressive defects and reduced food intake. Hence, skin sections from young (P3) mice were analyzed (B-I). B: Hematoxylin and eosin stained transgenic mouse skin section. Double arrows demarcate the border of mosaic histology, with seemingly normal epidermis (epi) and a mature hair follicle (hf) at left and hyperthickened epidermis at right. C: Immunofluorescence of transgenic mouse skin section labeled with antibodies as color-coded on frame. Double arrows demarcate the border of mosaic anti-Snail (green), revealing Snail expression coincident with regions of hyperthickened epidermis (at left) and absent in regions of normal epidermis (at right). D: Sections of P3 wild type or transgenic mouse skin (affected region) subjected to either immunofluorescence (D, E, H and I) or immunohistochemistry (F and G) with antibodies as indicated on the panel. Anti-keratin 5 indicates K5, normally restricted to the basal layer of the epidermis; anti-keratin 6 detects keratin 6, expressed in postnatal epidermis under conditions such as wounding, in which hyperproliferation occurs. Comparison of D and E provide representative examples that illustrate pMAPK is found in only a subset of all proliferating (Ki67-positve) cells. Note also the presence of Ki67- (E) and pMAPK-positive (G) cells in some suprabasal areas; Ki67-positive cells colabeled with anti-Snail (E).

Copyright

This image is from Jamora C, PLoS Biol 2005 Jan;3(1):e11, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:97750

Associated Alleles

Symbol	Name
Tg(KRT14-Snai1)1Efu	transgene insertion 1, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Tg(KRT14-Snai1)1Efu/0	involves: CD-1