Phenotype Image Detail

Image

Caption

A-H: Immunofluorescence of skin sections from P3 wild type (WT) and Tg(KRT14-Snai1)1Efu/0 (Tg) mice. Shown are affected areas of transgenic mouse skin; in areas where Snail protein was not expressed, stainings were normal. Sections were labeled with antibodies as indicated and color-coded on each frame. Antibodies are against markers of normal epidermal differentiation, and include K5 (a basally expressed keratin), K1 (a suprabasal keratin, expressed in spinous layer cells), involucrin (Inv; a suprabasally expressed cornified envelope protein found in upper spinous and granular layer cells), loricrin (Lor; a cornified envelope protein expressed in the granular layer), and filaggrin (Fil; a protein that bundles keratin filaments in the granular layer and stratum corneum). Note abnormal extension of anti-K5 suprabasally, often present in anti-K1 positive suprabasal transgenic mouse cells. I-N: Immunohistochemistry (I and J) or immunofluorescence (K-N) of sections of P30 wild type (I, K, and M) and transgenic (J, L, and N) (affected areas) mouse skins using the antibodies indicated. Note that with age, affected areas of the transgenic epidermis became increasing undulating, often exhibiting papilloma-like invaginations (J). Insets in I and J are magnified views of the boxed areas, illustrating the absence (WT) or presence (Tg) of nuclear anti-cyclin D staining. With age, affected areas of the transgenic mouse epidermis also displayed perturbations within the basement membrane, as judged by antibody labeling against either basement membrane (K and L) or hemidesmosomal (M and N) components. Double arrows in L demarcate mosaic zones, revealing that perturbations were restricted to hyperthickened, i.e., Snail-positive zones (to left of double arrows).

Copyright

This image is from Jamora C, PLoS Biol 2005 Jan;3(1):e11, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:97750

Associated Alleles

Symbol	Name
Tg(KRT14-Snai1)1Efu	transgene insertion 1, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Tg(KRT14-Snai1)1Efu/0	involves: CD-1