Phenotype Image Detail

Image

Caption

STAT3 deficiency during hematopoiesis results in Crohn's disease-like pathology. (A-J) Ulcers (arrow 1), transmural infiltration (arrow 2), and granuloma-like structures (arrow 3) in the ileocecal area of Stat3^tm1Flv/Stat3^tm1Flv Tg(Tek-cre)12Flv/0 (STAT3-CFF) mice in C, D and F-J. For comparison, the normal phenotype (STAT3-FF) is depicted in A, B, and E. (C and D) Ileum of mutant mouse. (E) Cecum of a control littermate and (F) of a mutant mouse. Magnifications: x40, A-D; x10, E and F. (G-J) Higher magnification views (x100, hematoxylin and eosin). (G)Crypt abscesses with necrotic neutrophils and monocytes (arrow 4), (H) high frequency of mitosis in the epithelium (arrow 5), (I) a marked infiltration of neutrophils, macrophages and eosinphils (arrow 6) and few lymphocytes, edema of the lamina propria, (J) epithelioid cells with eosinophilic cytoplasm (arrow 7) and other inflammatory cells in the lamina propria can be identified. (K) Intestinal mesentry of control and (L) mutant mice. Infiltration of neutrophils, macrophages, eosinphils (arrow 8 in L), and other cell types in the mesenteric fat tissue of the mutant mouse (x40, hematoxylin and eosin). Six sex-matched pairs of control and mutant littermates between 4 and 6 weeks of age were analyzed with similar results. IBD (inflammatory bowel disease) also spreads to the colon (N). Note bowel wall thickening with muscosal erosion (arrow 9) and transmural inflammation (arrow 10) in mutant mice (x40, hematoxylin and eosin).

Copyright

This image is from Welte T, Proc Natl Acad Sci U S A 2003 Feb 18;100(4):1879-84. Copyright 2003 National Academy of Sciences, U.S.A. J:81973

Associated Alleles

Symbol	Name
Stat3^tm1Flv	signal transducer and activator of transcription 3; targeted mutation 1, Richard A Flavell
Tg(Tek-cre)12Flv	transgene insertion 12, Richard A Flavell

Associated Genotypes

Allelic Composition	Genetic Background
Stat3^tm1Flv/Stat3^tm1Flv Tg(Tek-cre)12Flv/0	involves: 129 * C3H * C57BL/6