Phenotype Image Detail

Image

Caption

Histology of hearts from Tfam^tm1Lrsn/Tfam^tm1Lrsn Tg(Ckmm-cre)1Lrsn/0 (left) and their littermate controls (Tfam^loxP/Tfam^loxP) (right). Examples of immunoreactive cells are indicated by arrows. Trichrome stainings show no evidence for necrosis or fibrosis in mutant (A) or control (B) hearts. Double enzyme histochemical stainings for COX activity and succinate dehydrogenase activity show a mosaic loss of COX activity in mutant mouse hearts, as evidenced by the blue staining of cardiomyocytes (C), and normal COX activity in controls, as reflected by the brown staining of cardiomyocytes (D). TUNEL stainings demonstrate more TUNEL-positive cardiomyocytes in mutant hearts (E) than in control hearts (F). Immunohistochemical stainings of cleaved caspase 3 and cleaved caspase 7 show occasional positive cardiomyocytes in mutant hearts (G and I) and no staining in control hearts (H and J).

Copyright

This image is from Wang J, Proc Natl Acad Sci U S A 2001 Mar 27;98(7):4038-43. Copyright 2001 National Academy of Sciences, U.S.A. J:125471

Associated Alleles

Symbol	Name
Tfam^tm1Lrsn	transcription factor A, mitochondrial; targeted mutation 1, Nils-Goran Larsson
Tg(Ckmm-cre)1Lrsn	transgene insertion 1, Nils-Goran Larsson

Associated Genotypes

Allelic Composition	Genetic Background
Tfam^tm1Lrsn/Tfam^tm1Lrsn Tg(Ckmm-cre)1Lrsn/0	involves: 129S1/Sv * 129X1/SvJ