Phenotype Image Detail

Image

Caption

A-C: Alcian blue staining of E14.5 (A), E16.5 (B), and postnatal day 0 (C) growth plates. In wild type (WT) embryos, a growth plate has formed, composed of resting, proliferative, and hypertrophic zones. Bmpr1a^tm2.1Bhr/Bmpr1a^tm2.1Bhr Bmpr1b^tm1Kml/Bmpr1b^tm1Kml Tg(Col2a1-cre)1Bhr/? embryos (Bmpr1a^CKO; Bmpr1b^-/-) do not form an organized growth plate. Chondrocytes undergo random hypertrophy in mutants. D-F: Immunohistochemistry for type 2 collagen (D), link protein (E), and aggrecan (F). Expression of extracellular matrix (ECM) proteins is significantly reduced in mutant mice. Interestingly, some ECM production does take place at the peripheries of mutant condensations (Insets). res, resting zone; pro, proliferative zone; hyp, hypertrophic zone; oc, ossification center.

Copyright

This image is from Yoon BS, Proc Natl Acad Sci U S A 2005 Apr 5;102(14):5062-7. Copyright 2005 National Academy of Sciences, U.S.A. J:97410

Associated Alleles

Symbol	Name
Bmpr1a^tm2.1Bhr	bone morphogenetic protein receptor, type 1A; targeted mutation 2.1, Richard R Behringer
Bmpr1b^tm1Kml	bone morphogenetic protein receptor, type 1B; targeted mutation 1, Karen M Lyons
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer

Associated Genotypes

Allelic Composition	Genetic Background
Bmpr1a^tm2.1Bhr/Bmpr1a^tm2.1Bhr Bmpr1b^tm1Kml/Bmpr1b^tm1Kml Tg(Col2a1-cre)1Bhr/?	involves: 129S/SvEv * 129S7/SvEvBrd * C57BL/6 * SJL