Phenotype Image Detail

Image

Caption

Radiological and histological comparisons of wild type (Gsk-3B^+/+) and Gsk3b^tm1Jrw/Gsk3b⁺ (Gsk-3B^+/-) littermates at 12 weeks of age showing increased bone mass in the mutants. A: Plain X-ray of the whole body. B: Plain X-ray of the entire femur. C: 3D-CT image of the distal femur. D: von Kossa staining of the proximal tibia (bar, 200um) and toluidine blue staining of the growth plate indicated by the inset box above (bar, 20um). E: Histomorphometric analyses of bone volume and bone formation parameters in the proximal tibia. BV/TV, trabecular bone volume per tissue volume; C.Th, cortical thickness; Ob.S/BS, osteoblast surface per trabecular bone surface; Ob.S/B.Pm, osteoblast surface per trabecular bone perimeter; MAR, mineral apposition rate; BFR/BS, bone formation rate per trabecular bone surface. Lower right panel shows fluorescent micrographs of calcein-labeled mineralization fronts of the trabecular bones (bar, 10um). F: Histomorphometric analyses of bone resorption parameters in the proximal tibia. N.Oc/B.Pm, number of osteoclasts per 100mm of bone perimeter, Oc.S/BS, osteoclast surface per bone surface; ES/BS, eroded surface per bone surface. For (E) and (F), data are mean (bars)+/-SEM (error bars) of 10 mice per genotype. *P<0.05, **<0.01 vs. wild type. G: Formation of TRAP-positive multinucleated osteoclasts by the co-culture of calvarial primary osteoblasts (POB) and bone marrow macrophages (BMM) derived from either wild type or heterozygous mice. Representative pictures (left; bar, 200um) and the number of osteoclasts expressed as mean (bars) +/-SEM (error bars) of 8 wells per group.

Copyright

This image is from Kugimiya F, PLoS ONE 2007;2(9):e837, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:129364

Associated Alleles

Symbol	Name
Gsk3b^tm1Jrw	glycogen synthase kinase 3 beta; targeted mutation 1, James R Woodgett

Associated Genotypes

Allelic Composition	Genetic Background
Gsk3b^tm1Jrw/Gsk3b^+	involves: 129 * C57BL/6