Phenotype Image Detail

Image

Caption

A-D: Undecalcified sections of femurs stained with von Kossa stain and photographed at a magnification of 25. T, trabecular bone (red square); C, cortical bone (arrows). E-H: Primary spongiosa from wild type, Pth^tm1Dgo/Pth^tm1Dgo (PTH-/-), Pthlh^tm1Hmk/Pthlh^tm1Hmk (PTHrP-/-) and Pth^tm1Dgo/Pth^tm1Dgo Pthlh^tm1Hmk/Pthlh^tm1Hmk (PTH-/-, PTHrP-/-) mice stained with hematoxylin and eosin. I-L: Diaphyseal region from wild type and mutant mice stained with hematoxylin and eosin. M and N: The cortical thickness and trabecular bone volume were measured from each of the wild type, Pth^tm1Dgo/Pth^tm1Dgo, Pthlh^tm1Hmk/Pthlh^tm1Hmk and Pth^tm1Dgo/Pth^tm1Dgo Pthlh^tm1Hmk/Pthlh^tm1Hmk mice and presented as mean +/- SEM. O: The number of osteoblasts per field were counted in triplicate in the primary spongiosa of hematoxylin and eosin stained femurs of the mice and presented as mean +/- SEM. *P < 0.05, mutant mice relative to wild type mice. #P < 0.05, double mutant mice relative to Pth^tm1Dgo/Pth^tm1Dgo or Pthlh^tm1Hmk/Pthlh^tm1Hmk mice.

Copyright

This image is from Miao D, J Clin Invest 2002 May;109(9):1173-82 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:76407

Associated Alleles

Symbol	Name
Pthlh^tm1Hmk	parathyroid hormone-like peptide; targeted mutation 1, Henry M Kronenberg
Pth^tm1Dgo	parathyroid hormone; targeted mutation 1, David Goltzman

Associated Genotypes

Allelic Composition	Genetic Background
Pth^tm1Dgo/Pth^tm1Dgo	involves: 129S2/SvPas * C57BL/6J
Pth^tm1Dgo/Pth^tm1Dgo Pthlh^tm1Hmk/Pthlh^tm1Hmk	involves: 129S2/SvPas * C57BL/6J
Pthlh^tm1Hmk/Pthlh^tm1Hmk	involves: 129S2/SvPas * C57BL/6J