Phenotype Image Detail

Image

Caption

Uteri from typical wild type (A, +/+) and Itgb3^tm1Hyn/Itgb3^tm1Hyn (B, -/-) females that had been bred to homozygous mutant or wild type males, respectively. Dark patches in (B) indicate bleeding in the mutant uterus. (C and D) Heterozygous E16 embryos in utero, in wild type (C) or mutant (D) mothers. Some pups die in mutant mothers in utero, appearing pale, small and associated with a pale placenta (arrow). (E, F and H) Hematoxylin and eosin stained sections of placentae from mutant females mated with wild type males. Hematoxylin and eosin stained section of placenta from wild type female mated with mutant male (G). Arrowhead indicates severe hemorrhage observed within the labyrinth (E) and under the Reichert's membrane (F). (G) Labyrinth of wild type placenta; (H) Labyrinth of mutant mouse placenta where cell layers appear thick; this occurs in ~25% of mutant mouse placentae. (I and J) E16 embryos, wild type (I) and mutant (J). Note the hemorrhage in the mutant mouse muzzle. Arrow indicates hemorrhage in the skin. (K,L,M and N) After birth, some mutant mice suffer hemorrhage in the skin. Petechiae around the mouth of a 1 day old mutant pup (K) and hematoxylin and eosin stained section of this region indicating bleeding under the epidermis (I). Purpura on the forelimb of a 1 day old mutant pup (M) and hematoxylin and eosin stained section of this region indicating bleeding within the dermis (N). (O and P) Gut from newborn pups. Wild type (O) and mutant pup with actue GI hemorrhage (P). Scale bar: E, 400um; F, 50um; G, 70um.

Copyright

This image is from Hodivala-Dilke KM, J Clin Invest 1999 Jan;103(2):229-38 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:52262

Associated Alleles

Symbol	Name
Itgb3^tm1Hyn	integrin beta 3; targeted mutation 1, Richard Hynes

Associated Genotypes

Allelic Composition	Genetic Background
Itgb3^tm1Hyn/Itgb3^tm1Hyn	involves: 129S2/SvPas * C57BL/6