Phenotype Image Detail

Image

Caption

Histology and immunohistochemistry analyses of growth plates of P15 wild type (WT), Fgfr3^tm3.1Cxd/Fgfr3^tm3.1Cxd (369/369) and Fgfr3^tm3.1CxdFgfr3⁺ (369/+) mice at P15. A: Chondrocytes in the wild type growth plates are divided into 4 distinct zones, i.e. resting (Rc), proliferating (Pc), maturing (Mc) and hypertrophic (Hc) chondrocytes. B and C: In the mutant growth plates, the demarcation of each zone is not clear. Arrows point to the secondary ossification center. D-F: [3H] Thymidine incorporation. The labeled cells in wild type mark the Pc zone. In mutant growth plates, the labeled cells are fewer in number and scattered, suggesting that the majority of the cells are in a quiescent state. G-O: Immunohistochemical analysis of growth plates, using antibodies to FGFR3 (G-I), Stat5b (J-L, and M-O for higher magnification), and p19 (P-R). In all cases, the staining was the weakest in wild type, intermediate in heterozygous, and strongest in homozygous growth plates.

Copyright

This image is from Chen L, J Clin Invest 1999 Dec;104(11):1517-25 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:69849

Associated Alleles

Symbol	Name
Fgfr3^tm3.1Cxd	fibroblast growth factor receptor 3; targeted mutation 3.1, Chu-Xia Deng

Associated Genotypes

Allelic Composition	Genetic Background
Fgfr3^tm3.1Cxd/Fgfr3^tm3.1Cxd	involves: 129S6/SvEvTac
Fgfr3^tm3.1Cxd/Fgfr3^+	involves: 129S6/SvEvTac