Phenotype Image Detail

Image

Caption

Wild type (WT), Fgfr3^tm3.1Cxd/Fgfr3^tm3.1Cxd (369/369), and Fgfr3^tm3.1Cxd/Fgfr3⁺ (369/+) mice. Panels A-C, G-R are prepared from P15 mice and panels D-F from P1 mice. A-C: Enhanced TRAP staining at the interface between mutant hypertrophic chondrocytes and primary spongiosa (arrows); the mutant also showed less primary spongiosa. D-F: Alizarin Red S staining of undecalcified growth plates isolated from the knee joints of P1 mice. Arrows in (e) and (f) point to the advanced bone collars. G-O: In situ hybridization using probes for osteopontin (Op, g-i), osteonectin (On, j-l) and osteocalcin (Oc, m-o). P-R: Immunohistochemical staining of osteocalcin. Arrows in n and o point to ectopic expression of Oc in the maturing zone of chondrocytes. Both Op and On are also stronger in the heterozygous and homozygous trabecular bones.

Copyright

This image is from Chen L, J Clin Invest 1999 Dec;104(11):1517-25 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:69849

Associated Alleles

Symbol	Name
Fgfr3^tm3.1Cxd	fibroblast growth factor receptor 3; targeted mutation 3.1, Chu-Xia Deng

Associated Genotypes

Allelic Composition	Genetic Background
Fgfr3^tm3.1Cxd/Fgfr3^tm3.1Cxd	involves: 129S6/SvEvTac
Fgfr3^tm3.1Cxd/Fgfr3^+	involves: 129S6/SvEvTac