Phenotype Image Detail

Image

Caption

Eyes were dilated and examined by slit lamp in nonanesthetized mice. The dotted line shows the outline of the eye. A-C: 5 week old mice. A, wild type mice at age 5 weeks had clear lenses. B, Cryaa^tm1.1Ady/Cryaa⁺ mice at 5 weeks displayed low level opacities in the nuclear and posterior regions of the lens. C, Cryaa^tm1.1Ady/Cryaa^tm1.1Ady mice at 5 weeks displayed a severe opacity in the lens nucleus (stage 3) and the small eye phenotype. Note that the opacity lies in the center of the lens, whereas the cortical fibers are relatively clear. The opacities were digitally quantified using the ImageJ program. The light intensity at the cornea was normalized to 100. The plot profiles shown below the slit lamp images demonstrate a gradual increase in light scattering from the wild type to heterozygous to homozygous mice. D-F: 16 week old mice. D, wild type lens at 16 weeks. E, 16 week old heterozygous mouse lens had stage 2 lens opacity. F, 16 week old homozygous mouse displayed micro-ophthalmia with severe (stage 4) lens opacities. Note the dotted line showing that the homozygous eye is significantly smaller that the wild type and heterozygous littermates (D and E). The opacities were digitally quantified using the ImageJ program. The light intensity at the cornea was normalized to 100. The plot profiles shown below the slit lamp images demonstrate a gradual increase in light scattering from the wild type to heterozygous to homozygous mice.

Copyright

This image is from Xi JH, J Biol Chem 2008 Feb 29;283(9):5801-14 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:132296

Associated Alleles

Symbol	Name
Cryaa^tm1.1Ady	crystallin, alpha A; targeted mutation 1.1, Usha P Andley

Associated Genotypes

Allelic Composition	Genetic Background
Cryaa^tm1.1Ady/Cryaa^+	involves: 129 * C57BL/6
Cryaa^tm1.1Ady/Cryaa^tm1.1Ady	involves: 129 * C57BL/6