Phenotype Image Detail

Image

Caption

Organization of granule cells (B-D), Purkinje cells (A,E-G), and astroglia (H-J) in normal (A) and mea/mea (B-J) mature cerebellum. A: Normal cerebellar cortex immunostained with antisera to calbindin (28-kDa calcium-binding protein) for comparison to B-J. Note the regularity of thickness of the Purkinje cell monolayer in every folium. Purkinje cell dendrites are oriented toward the pia and fill the molecular layer (white bracket). The thin arrow in B, E and H indicates point of transition zone in mutant cerebellum, separating posterior (to right) from anterior (to left) poles. In B, E and H, normal posterior lobe folia are indicated by an open star corresponding to the high-power views in C, F and I. Defective anterior lobe folia are indicated by filled circle/star, corresponding to high-power views shown in D, G and J. B-D: Nissl-stained mutant cerebellum showing granule neurons. The internal granule cell layer, normally filled with numerous small darkly stained cells (C, arrow), rapidly disintegrates from the middle of the declive (left of arrow, B) to the anterior-most folium (filled circle/star in B and D). E-G: Purkinje cells immunostained with antisera to calbindin are normal to the right of the line at the declive (arrow, E) and are in a monolayer (arrow, F), and their dendrites project upward to fill the molecular layer (compare A to E and F at open star). In the most affected folia (white asterisk, E and G), Purkinje cell dendrites and cell bodies are disoriented, filling the merged folia. H-J: Astroglia immunostained with antisera to glial filament protein. Bergmann glia are normally regularly spaced, span the Purkinje and molecular layers, and project fibers radially, perpendicular to the pia (open star, H and I). In the most severely affected folia (star/circle, H and J), glial fibers are short, disoriented, and reduced in density (J) and, like the Purkinje cells in this area (E and G), fill the folium. Scale Bar in A for A,B,E and H, 1mm; bar in J for C,D,F,G,I and J, 50um. All are midline sagittal sections.

Copyright

This image is from Ross ME, Proc Natl Acad Sci U S A 1990 Jun;87(11):4189-92. Copyright 1990 National Academy of Sciences, U.S.A. J:28105

Associated Alleles

Symbol	Name
mea	meander tail

Associated Genotypes

Allelic Composition	Genetic Background
mea/mea	involves: STOCK Tyrp1^b Tyr^c-ch Mitf^Mi a^t Adamts20^bt