Phenotype Image Detail

Image

Caption

A and B: Hematoxylin and eosin staining of sagittal brain sections from wild type (+/+, A) and Hspg2^tm1Ref/Hspg2^tm1Ref (-/-, B) E11.5 embryos. Note the extension and the thinning of the anterior part of the forebrain in the mutant embryo (upper box in B). Ectopias are visible ventral of the medial ganglionic eminence (arrow) in the mutant embryo. C-F: Higher magnification of the lower boxes indicated in A and B. In the normal brain, the neuroepithelium and the underlying mesenchyme are separated by a basement membrane (BM) (C) that contains laminin-1 (E). In the mutant brain, the BM is discontinuous (D, arrow) and shows interrupted laminin-1 staining (F, arrow). G and H: Higher magnification of the upper boxes indicated in A and B. In a normal brain, the neuroepithelium and the overlying mesenchyme are separated by a BM (G). In the mutant brain, neuroepithelial cells have invaded the overlying ectoderm (H, arrow). I-L: Immunohistochemical localization of nestin and beta-tubulin type III in the neocortex of normal and mutant embryos. The neocortex of the wild type embryo contains nestin-positive cells (I) and a few beta-tubulin type III-positive cells in the cortical subplate (K). Ectopic cells in the mutant embryo are positive for nestin (J) but negative for beta-tubulin type III (L). M: Hematoxylin and eosin staining of the forebrain region of an E11.5 mutant showing a defect in the ectoderm and a small hole of 5-10um (arrow). Note that the amniotic membrane is still intact (arrrowhead). N-Q: Nissl staining of the coronal sections of wild type (N and P) and mutant (O and Q) E17.5 forebrain regions. The mutant brain shows a ruffled surface, large ectopias, and abnormal lamination (O). Posterior to this area, the ruffles and defects in lamination are less severe (Q). Abbreviations: mz, marginal zone; cp, cortical plate; iz, intermediate zone; and vz, ventricular zone. Scale bars: C-L, 125um; M, 250um; N-Q, 500um.

Copyright

This image is from Costell M, J Cell Biol 1999 Nov 29;147(5):1109-22, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:58700

Associated Alleles

Symbol	Name
Hspg2^tm1Ref	perlecan (heparan sulfate proteoglycan 2); targeted mutation 1, Reinhard Fassler

Associated Genotypes

Allelic Composition	Genetic Background
Hspg2^tm1Ref/Hspg2^tm1Ref	involves: 129S1/Sv * 129X1/SvJ