Phenotype Image Detail

Image

Caption

A-D: Hematoxylin and eosin staining of the coronal brain sections from wild type (+/+, A and C) and Hspg2^tm1Ref/Hspg2^tm1Ref (-/-, B and D) E12.5 embryos. Ectopias (B and D, arrows) are visible in the ventral forebrain of mutants. E and F: Cells within the ectopias are beta-tubulin type III-positive (F, arrow). G-L: Hematoxylin and eosin and immunostaining of sagittal brain sections from wild type (G, I and K) and mutant (H, J and L) E13.5 embryos. Ectopias (H,J and L, arrows) are visible in the ventral forebrain. Laminin-1 is expressed in the leptomeninges and around the capillaries in both the wild type (I) and mutant (J) brain. In mutants, laminin-1 staining is disrupted around the ectopias (arrows). Perlecan is expressed around capillaries and in the leptomeninges but not in brain of normal embryos (K). No perlecan staining is detectable in mutants (L). Scale bars: C-F and I-L, 250um.

Copyright

This image is from Costell M, J Cell Biol 1999 Nov 29;147(5):1109-22, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:58700

Associated Alleles

Symbol	Name
Hspg2^tm1Ref	perlecan (heparan sulfate proteoglycan 2); targeted mutation 1, Reinhard Fassler

Associated Genotypes

Allelic Composition	Genetic Background
Hspg2^tm1Ref/Hspg2^tm1Ref	involves: 129S1/Sv * 129X1/SvJ