Phenotype Image Detail

Image

Caption

Comparative histological analysis of developing vertebral colums from wild type and Col2a1^tm1Prc/Col2a1^tm1Prc mice. Sagittal sections of the cervical vertebral column (E12.5-17.5 and newborn [NB]) from wild type (+/+, A,C,E,G and I) and Col2a1^tm1Prc/Col2a1^tm1Prc (-/-, B,D,F,H and J) mice were stained with hematoxylin and eosin. The sections are presented at same scale to show the size differences between normal and mutant vertebral bodies. The orientation of I and J are at right angles to the others. The segmental differentiation of prevertebrae (pv) and intervertebral mesenchymes (im) were similar at E12.5 between wild type (A) and mutant embryos (B). In wild type mice, the notochord gradually expanded at the intervertebral disc areas to form a nucleus pulposus (np) and vanished within the vertebral bodies (vb) (C,E, G and I). In mutant mice, the rod-like structure of the notochord remained relatively unchanged, without any notable differential expansion or regression (D,F,H and J). Vertebral bodies of mutant mice became double in size. Other landmarks, such as prevertebrae (pv), intervertebral mesenchyme (im), notochord (n), inner annulus (ia), and notochordal tube. The arrows identify the notochordal sheath and asterisks indicate the acellular notochordal tube. Bar, 100um.

Copyright

This image is from Aszodi A, J Cell Biol 1998 Nov 30;143(5):1399-412, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:51376

Associated Alleles

Symbol	Name
Col2a1^tm1Prc	collagen, type II, alpha 1; targeted mutation 1, Darwin J Prockop

Associated Genotypes

Allelic Composition	Genetic Background
Col2a1^tm1Prc/Col2a1^tm1Prc	involves: 129/Sv * C57BL/6