Phenotype Image Detail

Image

Caption

Normal wild type (a,c and e) and Upk3a^tm1Tsun/Upk3a^tm1Tsun mouse bladders (b,d and f), all from 2-3 month old mice, were examined using hematoxylin and eosin-stained paraffin sections (a and b), or using toluidine blue-stained plastic thin sections (c and d). Appearance of methylene blue-stained wild type (e) and mutant (f) mouse bladders. G: Retention of methylene blue by wild type (n=7) and mutant mouse (n=5) bladder (P=0.019). The dye that was retained by the bladder was solubilized with chloroform and read at 660 nm. Note the rugged luminal surface of the normal, superficial umbrella cells (arrows), and the markedly different mutant urothelium, which was characterized by a smooth, apical cell surface (arrowheads), the absence of an umbrella cell layer, and the leakiness/retention of methylene blue. L, bladder lumen; M, mesenchyme; U, urothelium. Scale bars: a and b, 50um; c and d, 25um.

Copyright

This image is from Hu P, J Cell Biol 2000 Nov 27;151(5):961-72, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:77653

Associated Alleles

Symbol	Name
Upk3a^tm1Tsun	uroplakin 3A; targeted mutation 1, Tung-Tien Sun

Associated Genotypes

Allelic Composition	Genetic Background
Upk3a^tm1Tsun/Upk3a^tm1Tsun	either: (involves: 129S/SvEv) or (involves: 129S/SvEv * Swiss Webster)