Phenotype Image Detail

Image

Caption

A: Macroanatomy of inner ear structures dissected from E18.5 wild type (Vangl1^+/+), Vangl1^Gt(XL802)Byg/Vangl1^Gt(XL802)Byg (Vangl1^gt/gt), Vangl1^Gt(XL802)Byg/Vangl1⁺ (Vangl1^gt/+), Vangl1^Gt(XL802)Byg/Vangl1⁺ Vangl2^Lp/Vangl2⁺ (Vangl1^gt/+; Vangl2^lp/+) and Vangl2^Lp/Vangl2^Lp (Vangl2^lp/lp). B: Whole-mount preparations of organs of Corti from E18.5 wild type (WT), Vangl2^Lp/Vangl2^Lp, and Vangl1^Gt(XL802)Byg/Vangl1⁺ Vangl2^Lp/Vangl2⁺ embryos stained with phalloidin-FITC antibody to visualize actin-based stereociliary bundles. The analyzed regions were determined relative to the length of the cochlear duct. The base region was 5% from the most basal position of the chochlea, the 50% region was the midpoint between the most basal and most apical ends, and the apical region was at 75% from the most basal position. Each microscopic image is schematically depicted: White circles correspond to the hair cells with normal planar orientation of the stereociliary bundles, and the black circles correspond to the hair cells with randomized orientation of the actin vertexes. The individula layers of inner hair cells (IHC) and outer hair cells (OHC) (OHC-3) are identified. C: Quantification of the number of misoriented hair cells (as a perentage) found in embryos of different Vangl1 and Vangl2 genotypes. The asterisks indicate the level of significance (X² analysis): ***, P < 0.0005; **, P > 0.0005 to P < 0.005; *, P > 0.005 to P < 0.05. The absence of an asterisk indicates lack of significance (P > 0.05).

Copyright

This image is from Torban E, Proc Natl Acad Sci U S A 2008 Mar 4;105(9):3449-54. Copyright 2008 National Academy of Sciences, U.S.A. J:132697

Associated Alleles

Symbol	Name
Vangl1^Gt(XL802)Byg	VANGL planar cell polarity 1; gene trap XL802, BayGenomics
Vangl2^Lp	VANGL planar cell polarity 2; loop tail

Associated Genotypes

Allelic Composition	Genetic Background
Vangl2^Lp/Vangl2^Lp	LPT/LeJ
Vangl1^Gt(XL802)Byg/Vangl1^+ Vangl2^Lp/Vangl2^+	involves: 129P2/OlaHsd * C57BL/6J * LPT/LeJ