Phenotype Image Detail

Image

Caption

A: Subtle delay of fifth middle phalanx ossification in newborn Lrp5^tm1Kry/Lrp5^tm1Kry mice. B: Delayed ossification of metacarpals. C: Carpals (bracket). D: Humeri in 4 day old mutant mice. Arrows in A, B and D indicate ossification centers. A subtle delay was also evident in 44% (8/18) of Lrp5^tm1Kry/Lrp5⁺ mice (D, middle). E: Sections of calvaria from 4 day old mice showing a decreased number of BrdU-positive cells (arrowhead) in homozygous mutant mice compared with wild type littermates. Double-headed arrows indicate the greater thickness of wild type calvarium. F: Osteoblast mitotic index (percentage of BrdU-positive cells per total cell number) in wild type and homozygous mutant mice (n = 8 per genotype). G: Quantification of allkaline phosphatase positive stromal cell progenitors (CFU-F) in the bone marrow of wild type and homozygous mutant mice (n=9 mice per genotype). H: Quantification of apoptotic cells by TUNEL assay in wild type and homozygous mice (n = 5 per genotype). I: Northern blot analysis of Cbfa1 expression in calvaria, long bones, and primary osteoblasts. J: Northern blot analysis of Osteocalcin expression in calvaria and long bones. I and J: Gapdh expression level was used as a loading control; results are representative of three samples (n = 3 mice per sample). Asterisks indicate a statistically significant difference between two groups of mice (p<0.05). Error bars represent SD.

Copyright

This image is from Kato M, J Cell Biol 2002 Apr 15;157(2):303-14, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:75973

Associated Alleles

Symbol	Name
Lrp5^tm1Kry	low density lipoprotein receptor-related protein 5; targeted mutation 1, Gerard Karsenty

Associated Genotypes

Allelic Composition	Genetic Background
Lrp5^tm1Kry/Lrp5^tm1Kry	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
Lrp5^tm1Kry/Lrp5^+	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)