Phenotype Image Detail

Image

Caption

A: Representative hematoxylin and eosin stained cross-sections of an E16.5 Myh10^{tm6(Myh9/GFP)Rsad}/Myh10^{tm6(Myh9/GFP)Rsad} (B^a*/B^a*) mouse heart showing ventricular septal defect (VSD, yellow arrow in right panel) which is not seen in wild type (B⁺/B⁺) mice. RA, right atrium; LA, left atrium; RV, right ventricle; LV, left ventricle; VS, ventricular septum. Scale bar, 0.4 mm. B: Representative image of binucleated and enlarged cardiac myocytes in an E13.5 mutant mouse heart. Yellow arrows in the mutant heart indicate binucleated cardiac myocytes. Scale bar, 50 um. C: Representative flow cytometry profiles of cells isolated from E16.5 hearts. The cells were stained with an anti-alpha-actinin antibody to identify the cardiac myocytes (y axis). The percentages of cardiac myocytes and non-cardiac myocytes in wild type mice are 64.6 +/- 1.3 and 35.4 +/- 1.3% (mean +/- S.E., n = 4), respectively; whereas in mutant mice, the percentage is 38.0 +/- 6.9% for cardiac myocytes and 62.0 +/- 6.9% for non-cardiac myocytes (mean +/- S.E., n =6). Note that the GFP signal is detected in both myocytes and non-myocytes in the hearts of mutant mice (x axis).

Copyright

This image is from Bao J, J Biol Chem 2007 Jul 27;282(30):22102-11 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:124610

Associated Alleles

Symbol	Name
Myh10^tm6(Myh9/GFP)Rsad	myosin, heavy polypeptide 10, non-muscle; targeted mutation 6, Robert S Adelstein

Associated Genotypes

Allelic Composition	Genetic Background
Myh10^tm6(Myh9/GFP)Rsad/Myh10^tm6(Myh9/GFP)Rsad	involves: 129S6/SvEvTac * C57BL/6