Phenotype Image Detail

Image

Caption

A: Whole-mount phalloidin staining of the eyelids of wild type (WT, top) and Rock1^tm1Sna/Rock1^tm1Sna (KO, bottom) embryos at 15.5 and 16.0 dpc. Bidirectional arrows indicate extension of the eyelid epithelial sheet. Bar, 500um. B-G: Higher magnification views of the boxed regions in A. Arrowheads indicate actin cables in the eyelid epithelial sheet of wild type embryos. Bidirectional arrows indicate extension of the eyelid epithelial sheet. Mutant embryos failed to assemble continuous actin cables. Bar, 50um. H: Phalloidin staining of transverse sections of the eyelid rim of wild type (top) and mutant (bottom) embryos from 14.5 to 16.0 dpc. A substantial number of actin cables were apparent in a few layers of the eyelid epithelial sheet of wild type embryos (arrows). Little filamentous-actin accumulation was detected in mutant embryos, with the exception of a few actin bundles in some embryos (arrowhead). Bar, 50um. I: Immunofluorescence staining for phosphorylated MLC (green) and phalloidin staining (red) in sections of the eyelid of wild type (top) and mutant (bottom) embryos at 16.0 dpc. Phosphorylated MLC was enriched in the eyelid epithelial sheet of wild type embryos (arrowhead), where it was colocalized with actin cables (arrow). Little phosphorylated MLC staining was detected in mutant embryos. Bar, 50um. J: Immunofluorescence staining for ROCK-1 in sections of the eyelid (left) and skin (right) of wild type (top) and mutant (bottom) embryos at 16.0 dpc. Nonspecific signals are indicated by arrows. Bars, 50um.

Copyright

This image is from Shimizu Y, J Cell Biol 2005 Mar 14;168(6):941-53, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:98250

Associated Alleles

Symbol	Name
Rock1^tm1Sna	Rho-associated coiled-coil containing protein kinase 1; targeted mutation 1, Shuh Narumiya

Associated Genotypes

Allelic Composition	Genetic Background
Rock1^tm1Sna/Rock1^tm1Sna	B6N.129X1-Rock1^tm1Sna