Phenotype Image Detail

Image

Caption

A: Omphalocele in Rock1^tm1Sna/Rock1^tm1Sna embryos. Wild type and mutant embryos at 18.5 dpc are shown. Severe, moderate, and mild forms of omphalocele are apparent in the mutant mice shown in the center left, center right and right panels, respectively. Arrowheads indicate the epidermal ridge in the wild type embryo. B: Hematoxylin and eosin staining of sagittal sections through the umbilical region of wild type (top) and mutant (bottom) embryos at 15.5 (left) and 16.5 (right) dpc. Boxes indicate the region of the ectodermal-amnion transition. Arrows indicate the epidermal ridge. Bar, 1mm. C: Higher magnification images of the boxed regions in B. Arrows indicate the epidermal ridge in the wild type embryo. Bar, 200um. D: Staining of the epidermal ridge. Sections of the epidermal ridge in wild type (top) and mutant (bottom) embryos at 16.5 dpc were stained with an antibody to phosphorylated MLC (green, arrowheads) and phalloidin (red, arrows) in the left panels, and with phalloidin (red) and an antibody to keratin 5 (green) in the right panel. Bars, 50um.

Copyright

This image is from Shimizu Y, J Cell Biol 2005 Mar 14;168(6):941-53, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:98250

Associated Alleles

Symbol	Name
Rock1^tm1Sna	Rho-associated coiled-coil containing protein kinase 1; targeted mutation 1, Shuh Narumiya

Associated Genotypes

Allelic Composition	Genetic Background
Rock1^tm1Sna/Rock1^tm1Sna	B6N.129X1-Rock1^tm1Sna