Phenotype Image Detail

Image

Caption

Nerves were fixed with a fixative containing 0.1% tannic acid to clearly visualize the kissing points of tight junctions (TJ). A: Transverse sectional electron microscopic view of wild type (+/+) myelinated axons. Outer and inner mesaxons (boxed areas) are shown in B and D, respectively. Ax, axon; My, myelin sheath. B and B': Ultrathin section electron microscopy of outer mesaxons. In wild type myelinated axons, approximately one to three kissing points of TJs (arrowheads) were observed, whereas the outer mesaxons of Cldn19^tm1Sts/Cldn19^tm1Sts (-/-) myelinated axons completely lacked these kissing points, leaving widened intercellular gaps between apposed Schwann cell membranes (arrow). Asterisks, outer mesaxons, C and C': Freeze-fracture electron microscopy of outer mesaxons. Consistent with the images in B and B', TJ strand-like structures (arrowheads) were frequently observed at the outer mesaxon (asterisks) of wild type axons, but never mutant axons (arrow). D and D': Ultrathin section electron microscopy of inner mesaxons. The inner mesaxon of wild type myelinated axons (asterisk) usually bore one kissing point of TJs (arrowhead), whereas in the inner mesaxons of mutant myelinated axons, typical kissing points were never detectable (arrow). Bars: A, 200nm; B and B', 100nm; C and C', 200nm; D and D', 100nm,

Copyright

This image is from Miyamoto T, J Cell Biol 2005 May 9;169(3):527-38, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:99659

Associated Alleles

Symbol	Name
Cldn19^tm1Sts	claudin 19; targeted mutation 1, Shoichiro Tsukita

Associated Genotypes

Allelic Composition	Genetic Background
Cldn19^tm1Sts/Cldn19^tm1Sts	involves: 129S4/SvJae * C57BL/6