Phenotype Image Detail

Image

Caption

Loss of adrenomedullin signaling results in hypoplastic lymph sacs. Lymphs sacs of Calcrl^tm1Kmca/Calcrl^tm1Kmca (calcrl^-/-), Adm^tm1Unc/Adm^tm1Unc (AM^-/-), and Ramp2^tm1Kmca/Ramp2^tm1Kmca (RAMP2^-/-) embryos were significantly smaller than those of their wild type (WT) littermates at E13.5. A-D: Hematoxylin and eosin stained sections through transverse sections of the jugular lymph sacs of wild type (A), Calcrl^tm1Kmca/Calcrl^tm1Kmca (B), Adm^tm1Unc/Adm^tm1Unc (C), and Ramp2^tm1Kmca/Ramp2^tm1Kmca (D) embryos at E13.5. Note the remarkable decrease in size of lymph sacs relative to jugular vein and carotid artery in the adrenomedullin (AM) signaling-mutant embryos, despite massive interstitial edema. E-G: Quantitation of lymph sac area, normalized to jugular vein are in Calcrl^tm1Kmca/Calcrl^tm1Kmca (E), Adm^tm1Unc/Adm^tm1Unc (F), and Ramp2^tm1Kmca/Ramp2^tm1Kmca (G) embryos (black bars) and their wild type littermates (gray bars) at E13.5. E,F and G have different scales. n>6 embryos per genotype. Error bars indicate SEM. Original magnification x200.

Copyright

This image is from Fritz-Six KL, J Clin Invest 2008 Jan;118(1):40-50 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:130930

Associated Alleles

Symbol	Name
Adm^tm1Unc	adrenomedullin; targeted mutation 1, University of North Carolina
Calcrl^tm1Kmca	calcitonin receptor-like; targeted mutation 1, Kathleen M Caron
Ramp2^tm1Kmca	receptor (calcitonin) activity modifying protein 2; targeted mutation 1, Kathleen Caron

Associated Genotypes

Allelic Composition	Genetic Background
Calcrl^tm1Kmca/Calcrl^tm1Kmca	129S6/SvEvTac-Calcrl^tm1Kmca
Ramp2^tm1Kmca/Ramp2^tm1Kmca	involves: 129S6/SvEvTac * C57BL/6
Adm^tm1Unc/Adm^tm1Unc	129S6/SvEvTac-Adm^tm1Unc