Phenotype Image Detail

Image

Caption

A comparison between newborn wild type (+/+, a) and Lmx1b^tm1Rjo/Lmx1b^tm1Rjo (-/-, b) mice demonstrates the severely retarded outgrowth of glomerular capillaries when the Lmx1b gene is inactiviated. Furthermore, a fibrillar material can be detected in Bowman's space and adjoining proximal tubules of mutant mice (asterisk in b). At higher magnification, the reduction and sometimes even complete lack of fenestrae in the endothelium can be seen in the mutant mice (d). (arrows in c point to fenestrae in the glomerular endothelium of wild type mice). Although by in situ hybridization VEGF mRNA can be detected in the podocytes of both wild type (e) and mutant (f) mice, an RNase protection assay with 20 ug of total kidney RNA (prepared from five wild type and four mutant mice) demonstrates reduced levels of VEGF mRNA in kidneys from the mutant mice (tRNA served as a negative control). A protection assay with a probe directed against 18S rRNA shows that the RNA concentration was determined correctly (g). Bar: a,b e and f, 20um; c, 0.5um; d, 4um.

Copyright

This image is from Rohr C, J Clin Invest 2002 Apr;109(8):1073-82 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:76088

Associated Alleles

Symbol	Name
Lmx1b^tm1Rjo	LIM homeobox transcription factor 1 beta; targeted mutation 1, Randy L Johnson

Associated Genotypes

Allelic Composition	Genetic Background
Lmx1b^tm1Rjo/Lmx1b^tm1Rjo	involves: C57BL/6