Phenotype Image Detail

Image

Caption

A-D: Hematoxylin and eosin staining of embryonic 18.5 dpc lungs. Embryonic lungs from wild type (WT, A and C) and Foxm1^tm1.1Rhc/Foxm1^tm1.1Rhc (Foxm1-/-, B and D) 18.5 dpc embryos were fixed, paraffin-embedded, sectioned and then H&E stained. Pulmonary arteries (Ar) of the mutant embryos displayed severe hypertrophy of vascular smooth muscle cells (D). E-H: Immunohistochemical staining of lung sections with antibodies specific to Plk-1 (G-H) or Aurora B proteins (E-F). Paraffin section from wild type (E and G) and mutant (F and H) embryos were stained with primary antibody and then visualized by anti-mouse antibody conjugated with alkaline phosphatase and BCIP/NBT substrate followed by counterstaining with nuclear fast red. I: Increased muscularity of mutant arteries. H&E stained sections were used to measure the thickness of smooth muscle layers in wild type or mutant 18.5 dpc pulmonary arteries (arrowheads in C and D) with vessel diameter 100-300 um. Mean +/- S.D. was calculated using ten arteries in three different wild type or mutant embryos. J: Decreased numbers of Plk-1-positive and Aurora B-positive cells in mutant lungs. Plk-1 or Aurora B-stained lung sections were used to count the number of cells with positive immunostaining in five random x400 microscope fields. Three different WT or mutant lungs were used to calculate the mean S.D. A p value greater to or equal to 0.05 is shown with an asterisk. Abbreviations: Br, bronchi. Magnifications: A and B, x100; C-F, x400; G and H, x200.

Copyright

This image is from Kim IM, J Biol Chem 2005 Jun 10;280(23):22278-86 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:100263

Associated Alleles

Symbol	Name
Foxm1^tm1.1Rhc	forkhead box M1; targeted mutation 1.1, Robert H Costa

Associated Genotypes

Allelic Composition	Genetic Background
Foxm1^tm1.1Rhc/Foxm1^tm1.1Rhc	involves: 129X1/SvJ * C57BL/6