Phenotype Image Detail

Image

Caption

A-D: Hematoxylin and eosin (H&E) staining of 15.5 dpc mouse lungs. Mouse lungs were dissected from wild type (WT, A and C) and Foxm1^tm1.1Rhc/Foxm1^tm1.1Rhc (Foxm1-/-, B and D) 15.5 dpc embryos and then were fixed and paraffin-embedded. Paraffin sections were stained with H&E. Mutant embryos displayed diminished numbers of mesenchymal cells surrounding distal pulmonary epithelium (ep, D). E-F: Mutant lungs exhibited defects in mesenchyme proliferation. Paraffin sections from wild type (E) and mutant (F) 15.5 dpc embryos were immunohistochemically stained with BrdUrd antibody followed by anti-mouse antibody conjugated with alkaline phosphatase and BCIP/NBT substrate. Lung sections were counterstained with nuclear fast red. Although BrdUrd was detected in both epithelial and mesenchymal cells of wild type lungs (E), mutant lungs exhibited only epitelial BrdUrd staining (F, shown with arrows). G-J: Immunostaining of lung sections with antibodies specific to Foxa2 and vimentin. Lung 15.5 dpc sections were stained for Foxa2 (G and H) and vimentin protein (I and J). Mutant and wild type lungs exhibited similar Foxa2 and vimentin staining. Magnifications: A-B and G-H, x100; C and D, x400; E-F and I-J, x200.

Copyright

This image is from Kim IM, J Biol Chem 2005 Jun 10;280(23):22278-86 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:100263

Associated Alleles

Symbol	Name
Foxm1^tm1.1Rhc	forkhead box M1; targeted mutation 1.1, Robert H Costa

Associated Genotypes

Allelic Composition	Genetic Background
Foxm1^tm1.1Rhc/Foxm1^tm1.1Rhc	involves: 129X1/SvJ * C57BL/6