Phenotype Image Detail

Image

Caption

Histological changes in basooccipital-basosphenoid synchondroses in 1.5 day old wild type and Ltbp3^tm1Bdvc/Ltbp3^tm1Bdvc mice. A and B: Weingart hematoxylin, Safranin O and Fast green staining. Wild type (A), Ltbp3^tm1Bdvc/Ltbp3^tm1Bdvc (B). Wider zone of hypertrophic chondrocytes in the mutant synchondrosis compared to the wild type indicates more extensive differentiation. Cartilage is stained red. h, hypertrophic chondrocyte zone; r, resting chondrocyte zone; p, proliferating chondrocyte zone; ph, prehypertrophic chondrocyte zone. C and D: Immunostaining for collagen X. (C) Wild-type. (D) Mutant. E and F: Immunostaining for collagen II. (E) Wild-type. (F) Mutant. G and H: Masson's trichrome staining for bone. More advanced bone fronts (blue) are apparent in mutant (H) versus wild-type (G) synchondrosis. Arrows point to fronts of the cortical basooccipital and basosphenoid bones. I and J: In situ hybridization for bone sialoprotein 1 (Bsp-1). (I) Wild-type. (J) Mutant. K and L: In situ hybridization for Ihh. (K) Wild-type. (L) Mutant. The strong signal in the middle of the mutant mouse synchondrosis (L) suggests that these chondrocytes are already committed to hypertrophic differentiation. M and N: In situ hybridization for PTH/PTHrP-R. Expression pattern is less defined in the mutant animal sample (N) compared to the wild-type (M), and the transcript is detected through the central region of the synchondrosis. The intensity of the signal is similar in wild-type and in mutant samples. O and P: In situ hybridization for PTHrP. In wild-type synchondroses (O) expression is apparent in proliferating chondrocytes and in lateral chondrocytes of the central region of the synchondrosis. The signal is absent in the resting chondrocytes in the center of synchondrosis. The intensity of the signal is decreased in mutant synchondrosis (P).

Copyright

This image is from Dabovic B, J Cell Biol 2002 Jan 21;156(2):227-32, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:76213

Associated Alleles

Symbol	Name
Ltbp3^tm1Bdvc	latent transforming growth factor beta binding protein 3; targeted mutation 1, Branka Dabovic

Associated Genotypes

Allelic Composition	Genetic Background
Ltbp3^tm1Bdvc/Ltbp3^tm1Bdvc	involves: 129S6/SvEvTac