Phenotype Image Detail

Image

Caption

C-G: Skeletal staining with Alzarin red and Alcian blue of E16.5 Ccn3^tm1Cabo/Nov⁺ (+/-), Ccn3^tm1Cabo/Ccn3^tm1Cabo (-/-) and wild type (+/+) embryos. C: Wild type forefoot showing ossification of digits (arrow). D: No ossification in homozygous mutant digits (arrow), but intense staining with Alizarin red of the radius and ulna indicating increased bone mineralization. E-G: Vertebrae and rib cages of wild type (E) homozygote (F) and heterozygote (G) showing delay in ossification of the vertebrae in the mutant embryos. No ossification is present in the homozygous mutant embryo, and whilst ossification is taking place in the heterozygous embryo (G), it is not occuring in the ordered, sequential manner observed in the wild type. Thoracic vertebrae numbered 1-13. H-Q: Histological sections of E16.5 wild type and homozygous mutant embryos. H,J,L,N and P: wild type; I,K,M,O and Q: homozygous mutant littermate. H and I: Hematoxylin and eosin stained sections showing expanded perichondrium and periosteum in homozygotes and a thicker bone collar (arrowhead) compared to the wild type. o, ossified bone; h, hypertrophic cartilage; ph, pre-hypertrophic cartilage; c, columnar chondrocytes. J and K: Hematoxylin and eosin stained sections at higher magnification at the junction of pre-hypertrophic chondrocytes, adjacent to the border of the perichondrium/periosteum, showing abnormal morphology of chondrocytes and matrix in the mutant (K). L and M: Alcian blue staining of cartilage, showing expansion of the cartilage element and blurring of its borders in the homozygous embryo (M) compared to wild type (L). N and O: PCNA staining showing a sharp demarcation (black dashed line) between proliferating chondrocytes and pre-hypertrophic chondrocytes in wild type (N), but not in the homozygous mutant (black dasked line) (O). P and Q: Von Kossa staining for mineralized bone showing a shorter and thicker bone collar (indicated by the black bar) in the pre-hypertrophic/hypertrophic zone of the homozygous mutant (Q) compared to wild type (P). Scale bars: H,I,N,O,P,Q, 10um; J-M, 5um.

Copyright

This image is from Heath E, BMC Dev Biol 2008;8():18, an open-access article, licensee BioMed Central Ltd. J:135676

Associated Alleles

Symbol	Name
Ccn3^tm1Cabo	cellular communication network factor 3; targeted mutation 1, Catherine A Boulter

Associated Genotypes

Allelic Composition	Genetic Background
Ccn3^tm1Cabo/Ccn3^+	involves: 129S/SvEv
Ccn3^tm1Cabo/Ccn3^tm1Cabo	involves: 129S/SvEv