Phenotype Image Detail

Image

Caption

A: Electron micrographs of sections from the quadriceps muscle of 8 week old male wild-type (WT) and Syt7^tm1Nan/Syt7^tm1Nan (KO) mice. In contrast to wild-type muscle (top left), in mutant mice, the collagen accumulation and infiltration of inflammatory cells is evident. Arrows point to bundles of collagen fibers, large arrowheads to eosinophils, and small arrowheads to macrophages; n indicates muscle fiber nuclei; c, capillaries; and m, mast cell. B: Detection of inflammatory cells in the skeletal muscle of mutant mice with specific antibodies. Frozen sections of quadriceps muscle of 8 week old male wild-type and mutant mice were incubated with biotinylated mAbs to CD3 (T cells), CD11b (macrophages and NK cells), or Ly6G (neutrophils and eosinophils), followed by streptavidin/peroxidase-conjugated secondary antibodies. The first two panels (stained for CD3 and CD11b, respectively) are serial sections, indicating that the same muscle fibers are simultaneously surrounded by T cells and macrophages.

Copyright

This image is from Chakrabarti S, J Cell Biol 2003 Aug 18;162(4):543-9, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:85201

Associated Alleles

Symbol	Name
Syt7^tm1Nan	synaptotagmin VII; targeted mutation 1, Norma W Andrews

Associated Genotypes

Allelic Composition	Genetic Background
Syt7^tm1Nan/Syt7^tm1Nan	involves: 129S1/Sv * C57BL/6