Phenotype Image Detail

Image

Caption

Vital staining with Evans blue dye (EBD) reveals disruption of membrane integrity in Sgcg^tm1Mcn/Sgcg^tm1Mcn (-/-) muscle. Mice were injected with EBD and euthanized after 12 hours. A: Low-power magnification (5x) of diaphragm muscle from EBD-injected wild-type (+/+) and mutant mice. Diaphragm muscle from mutant mice exhibits segmental EBD uptake (seen as blue areas) while no EBD uptake is seen in wild type diaphragm. B: Regions of EBD uptake are seen as red cytoplasmic staining on fluorescence microscopy. No evidence of EBD microscopic staining is seen in wild-type muscle. Left: A region of mutant triceps muscle with marked EBD staining (black bar, 100um). Right: A higher power view of mutant gastrocnemius muscle showing EBD staining (white bar, 25um). C: Apoptosis in mutant mouse muscle. TUNEL-labeling was performed on quadriceps muscle from a mutant mouse. TUNEL-positive nuclei appear green. No TUNEL-positive nuclei were found in wild-type myofibers (left). In contrast, myofibers with TUNEL-positive nuclei were seen commonly in mutant muscle (right). D: Counterstaining with an anti-dystrophin antibody (yellow-orange) demonstrates that TUNEL-positive nuclei are found within mutant myofibers. Myofibers undergoing degeneration and demonstrating decreased membrane staining for dystrophin also show evidence of apoptosis.

Copyright

This image is from Hack AA, J Cell Biol 1998 Sep 7;142(5):1279-87, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:49871

Associated Alleles

Symbol	Name
Sgcg^tm1Mcn	sarcoglycan, gamma (dystrophin-associated glycoprotein); targeted mutation 1, Elizabeth M McNally

Associated Genotypes

Allelic Composition	Genetic Background
Sgcg^tm1Mcn/Sgcg^tm1Mcn	involves: 129X1/SvJ * C57BL/6