Phenotype Image Detail

Image

Caption

Histological analysis of the oral mucosa in Krt6a/Krt6b^tm1Cou/Krt6a/Krt6b^tm1Cou mice. A-H: Hematoxylin and eosin staining of wild-type (+/+), heterozygous (+/-) and Krt6a/Krt6b^tm1Cou/Krt6a/Krt6b^tm1Cou (-/-) mice tongues. In wild-type mice, the upper palate exhibits normal epithelial differentiation (A) and the dorsal tongue shows well-developed filiform papillae (B). Similar results are found in heterozygous mice (C and D). In mutant mice, the upper palate contains large amounts of cellular debris facing the oral cavity (E). F, shows the transition zone between unaffected and affected dorsal tongue epithelium. Blisters (bl) form as a result of cleavage within the suprabasal layers of the epithelium. The tips of both wild-type and mutant mice tongues are similar at low magnification (G and H). I-P: Immunohistochemical staining of wild-type and mutant tongue. Antibodies to K6 shows reactivity in wild-type, but not in mutant mouse tongue sections (I and J). The immunostaining reaction in these samples was overdeveloped in an attempt to detect any residual K6 expression in mutant mice tongues. Antibodies to K5 stain the basal layer of wild-type tongues (K) while staining the basal and adjacent suprabasal cells in blistered dorsal epithelium (L). K4 antibody staining detects the interpapillary region in both types of samples. The regular staining pattern is severely disturbed in blistered epithelium (M and N). Wild-type staining for K17 shows a strong reactivity in the papillae (O), whereas reactivity adjacent to the blister is basal and restricted to the lower most portion of blistered epithelium (P). Bars: A-F, I-P, 200um; G and H, 100um.

Copyright

This image is from Wong P, J Cell Biol 2000 Aug 21;150(4):921-8, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:64033

Associated Alleles

Symbol	Name
Krt6a/Krt6b^tm1Cou	keratin 6B; targeted mutation 1, Pierre A Coulombe
Krt6a/Krt6b^tm1Cou	keratin 6A; targeted mutation 1, Pierre A Coulombe

Associated Genotypes

Allelic Composition	Genetic Background
Krt6a/Krt6b^tm1Cou/Krt6a/Krt6b^tm1Cou	involves: 129S2/SvPas * C57BL/6 * DBA/2
Krt6a/Krt6b^tm1Cou/Krt6a/Krt6b^tm1Cou	involves: 129S2/SvPas * C57BL/6 * DBA/2