Phenotype Image Detail

Image

Caption

Histological and histochemical analysis of skeletal muscles. Fresh frozen gastrocnemius muscle specimens from wild-type (A,D,G and J), Ldb3^tm2Chen/Ldb3^tm2Chen (Cypher2sKI, B,E,H and K) and Ldb3^tm3Chen/Ldb3^tm3Chen (Cypher3sKI, C,F,I and L) mice were stained with hematoxylin and eosin (A-C), modified Gomori trichrome stain (D-F), or reacted for myofibrillar ATPase, pH 4.3 (G-I) and cytochrome c oxidase (J-L) activity. Variability in myofiber size and numerous atrophic fibers were observed in Ldb3^tm2Chen/Ldb3^tm2Chen (B) and Ldb3^tm3Chen/Ldb3^tm3Chen (C) muscle when compared with wild-type littermates (A). The black arrows in Ldb3^tm2Chen/Ldb3^tm2Chen (B and E) and Ldb3^tm3Chen/Ldb3^tm3Chen (C and F) muscle indicate subsarcolemmal invaginations resulting in a lobulated pattern, which are basophilic with the hematoxylin and eosin stain and reddish with Gomori trichrome stain. The white arrow in Ldb3^tm3Chen/Ldb3^tm3Chen (F) indicates regions within the muscle tissue with a clear, hyaline appearance, which were associated with a loss of cytochrome C oxidase activity (L). Note that lobulations and translucent regions were not apparent in wild-type muscle (A,D,G and J). The atrophic fibers, lobulations, and clear areas were present in both muscle fiber types (I). Bar, 100um.

Copyright

This image is from Huang C, J Biol Chem 2003 Feb 28;278(9):7360-5 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:82141

Associated Alleles

Symbol	Name
Ldb3^tm2Chen	LIM domain binding 3; targeted mutation 2, Ju Chen
Ldb3^tm3Chen	LIM domain binding 3; targeted mutation 3, Ju Chen

Associated Genotypes

Allelic Composition	Genetic Background
Ldb3^tm2Chen/Ldb3^tm2Chen	involves: 129S1/Sv * 129X1/SvJ * Black Swiss
Ldb3^tm3Chen/Ldb3^tm3Chen	involves: 129S1/Sv * 129X1/SvJ * Black Swiss