Phenotype Image Detail

Image

Caption

Hematoxylin and eosin staining (A and B), immunohistochemical analysis for expression of ADAR (ADAR1) by using an ADAR-specific mAb (C and D) and for TUNEL assays (E-H) were conducted with serial sections of wild-type (+/+) and Adar^tm1.1Knk/Adar^tm1.1Knk (-/-) embryos recovered live at E11.0. For immunostaining analysis of mutant embryo sections (D), the final color development step was carried out for twice as long (10 min) as in the control experiment (C) done for wild-type embryo sections so that at least the outline of the mutant embryos can be seen. Otherwise, immunostaining of mutant embryo sections was in practice blank. By using the same ADAR-specific mAb, Western blot analysis was carried out for protein samples extracted from whole embryo bodies (E11.0), demonstrating the absence of both normal and aberrant ADAR protein products in mutant embryos (right panel). TUNEL assays carried out on whole embryos (E and F) and liver sections (G and H) reveal widespread apoptosis in tissues stained dark silver gray. The highest levels of apoptosis were detected in heart (ht), liver (lv) and vetebra (vt). Examples of apoptotic hepatocytes (black arrowheads) and hematopoietic cells (red arrowheads) are indicated (H).

Copyright

This image is from Wang Q, J Biol Chem 2004 Feb 6;279(6):4952-61 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:87714

Associated Alleles

Symbol	Name
Adar^tm1.1Knk	adenosine deaminase, RNA-specific; targeted mutation 1.1, Kazuko Nishikura

Associated Genotypes

Allelic Composition	Genetic Background
Adar^tm1.1Knk/Adar^tm1.1Knk	involves: 129S1/Sv * 129X1/SvJ