Phenotype Image Detail

Image

Caption

A-F: Whole mount immunohistochemical staining of E8.5-9.5 embryos using a mAb against the endothelium-specific marker PECAM-1. Differentiated endothelial cells were observed in wild-type (Foxo1(+/+), A) and Foxo1^tm1Tf/Foxo1^tm1Tf (Foxo1(-/-), B) embryos at E8.5, and the formation of the dorsal aorta was unaffected by the lack of Foxo1. Although the first and second aortic arch arteries were readily apparent in E9.5 wild-type embryos (C), there was no evidence of the aortic arch arteries arising from the aortic sacs in mutant embryos (D). In the head, the capillary bed appeared dilated in the mutant similar to the yolk sac phenotype (E) and apparently arrested at the primary plexus stage (F). G-N: Show abnormal vascular remodeling in the yolk sacs of mutant embryos. The wild-type yolk sac appeared to have a vasculature (G), but the mutant yolk sac was pale and had not clear vasculature (H). I-L: Whole mount immunohistochemical staining of E8.75 and E9.5 embryos using mAb against the endothelium-specific marker PECAM-1. A honeycomb-like vascular plexus was evident by E8.75 (I), and subsequent remodeling of the vasculature resulted in defined vessels by E9.5 (K). Mutant yolk sacs showed a honeycomb-like vascular plexus similar to that of wild-type yolk sacs at E8.75 (J), but had not developed a normal vasculature at E9.5 (L). Histological analysis by hematoxylin and eosin staining revealed that whereas capillary-like vessels containing blood cells with an endothelial cell lining were present in wild-type yolk sacs (M), no distinct blood vessels were evident in mutant yolk sacs (N). Scale bars: A and B, 160um; C-L, 200um; M and N, 50um.

Copyright

This image is from Furuyama T, J Biol Chem 2004 Aug 13;279(33):34741-9 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:92247

Associated Alleles

Symbol	Name
Foxo1^tm1Tf	forkhead box O1; targeted mutation 1, Tatsuo Furuyama

Associated Genotypes

Allelic Composition	Genetic Background
Foxo1^tm1Tf/Foxo1^tm1Tf	involves: C57BL/6J